Evaluation of transcutaneous immunization as a delivery route for an enterotoxigenic E. coli adhesin-based vaccine with CfaE, the colonization factor antigen 1 (CFA/I) tip adhesin
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چکیده
منابع مشابه
Analysis of colonization factor antigen I, an adhesin of enterotoxigenic Escherichia coli O78:H11: fimbrial morphology and location of the receptor-binding site.
Colonization factor antigen I (CFA/I) of enterotoxigenic Escherichia coli was dissociated into one type of subunit (15 kDa). The dissociation was achieved either by heating CFA/I in sodium dodecyl sulfate at 100 degrees C or by heating it for 20 min in water. Heating in water to 100 degrees C yielded only in the 15-kDa subunit, but heating to 85 degree C yielded small amounts of oligomers in ad...
متن کاملNew vaccine strategies against enterotoxigenic Escherichia coli. I: DNA vaccines against the CFA/I fimbrial adhesin.
Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen ...
متن کاملShear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli.
In the intestine, enterotoxigenic Escherichia coli works against peristaltic forces, adhering to the epithelium via the colonization factor antigen I (CFA/I) fimbrial adhesin CfaE. The CfaE adhesin is similar in localization and tertiary (but not primary) structure to FimH, the type 1 fimbrial adhesin of uropathogenic E. coli, which shows shear-dependent binding to epithelial receptors by an al...
متن کاملMaternal vaccination with a fimbrial tip adhesin and passive protection of neonatal mice against lethal human enterotoxigenic Escherichia coli challenge.
Globally, enterotoxigenic Escherichia coli (ETEC) is a leading cause of childhood and travelers' diarrhea, for which an effective vaccine is needed. Prevalent intestinal colonization factors (CFs) such as CFA/I fimbriae and heat-labile enterotoxin (LT) are important virulence factors and protective antigens. We tested the hypothesis that donor strand-complemented CfaE (dscCfaE), a stabilized fo...
متن کاملA RECEPTOR-BINDING SITE AS REVEALED BY THE CRYSTAL STRUCTURE OF CfaE, THE CFA/I FIMBRIAL ADHESIN OF ENTEROTOXIGENIC Escherichia coli
Address correspondence to: Di Xia, Laboratory of Cell Biology, National Cancer Institute, NIH, 37 Convent Dr., Building 37, Room 2122C, Bethesda, MD 20892, USA, Tel: (301) 435-6315; Fax: (301) 480-2315; Email: [email protected]; or Stephen J. Savarino, U.S. Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA, Tel: (301) 319-7650; Fax: (301) 319-7679; Email: savar...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2019
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2019.08.057